Biotechnology is used in the development of vaccine in three different ways: Using a special monoclonal antibody in the separation of pure antigens, using cloned genes for the synthesis of antigens, synthesized proteins used as vaccines. Recombinant vaccines are vaccines produced from recombinant DNA Technology. They are also called as subunit vaccines. It is necessary that the modern biopharmaceutical vaccines are rationally designed with chemical and physical attributes that distinguish the microbes and creates immune response.