The vaccines in which the desired genes that encode the antigen protein for the particular disease are inserted into the genome of plant tissue by various methods are termed plant-based vaccines. The most common methods that are used to produce effective plant-based vaccines are Agrobacterium-mediated gene transfer and Transformation via genetically modified plant virus. However, advancements in the field of science and technology developed new approaches such as agroinfiltration, biolistics, electroporation, polyethylene glycol treatment and sonication to replace the former methods.
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